Application

Dot Blot (0.1-0.5 µg/ml)r>ELISA (0.1-0.5 µg/ml)r>Radioimmunoassay (0.5 µg/ml)

General description

This Anti-β-Amyloid (1-42) Rabbit pAb is validated for use in Dot Blot, ELISA, Radioimmunoassay for the detection of β-Amyloid (1-42).

Rabbit polyclonal antibody adsorbed against Aβ_1-40 and Aβ_1-43, followed by immunoaffinity purification. Recognizes specifically β-amyloid peptide (1-42).

Recognizes specifically β-amyloid peptide (1-42). Does not cross-react with β-amyloid peptide (1-40) or β-amyloid peptide (1-43).Antibody Target Gene Symbol: APP Target Synonym: AAA, AD1, Adap, AL024401, Amyloid precursor, Amyloidogenic glycoprotein, APP isoform 1, APPI, appican, CTFgamma, CVAP, E030013M08RIK, Nexin II, P3, PN2, PreA4, PROTEASE NEXIN2 Entrez Gene Name: amyloid beta (A4) precursor protein Hu Entrez ID: 351 (Related Antibodies: 171610, PC729,PC152, PC151, PC149, NE1012,171609, 171608, 171607, 171606, 171605, 171604, 171603, 171598, 171537) Mu Entrez ID: 11820 Rat Entrez ID: 54226

Immunogen

a synthetic peptide corresponding to amino acids from a C-terminal fragment of human β-amyloid peptide (1-42)

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Savage, M.J., et al. 1998. J. Neurosci.15, 1743.Borchelt, D.R., et al. 1997. Neuron19, 939.Citron, M., et al. 1997. Nature Med.3, 67.Hardy, J. 1997. Trends in Neurosci.20, 154.Solomon, B., et al. 1997. Proc. Natl. Acad. Sci. USA94, 4109.Duff, K., et al. 1996. Nature383, 710.Iwatsubo, T., et al. 1994. Neuron13, 45.Suzuki, N., et al. 1994. Science264, 133.

This antibody is specific for β-amyloid peptide [1-42] and shows no reactivity to β-amyloid peptide [1-40] or β-amyloid peptide [1-43]. This antibody is recommended for use in the quantitative determination of β-amyloid peptide [1-42]. The sensitivity level for sandwich ELISA is 15-25 pg. Antibody should be titrated for optimal results in individual systems.

Packaging

25 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)